Resveratrol Xanthine Oxidase Inhibitor

Title: Antioxidant Activity and Protective Effect on DNA Cleavage of Resveratrol. Authors: R. Acquaviva, A. Russo, A. Campisi, V. Sorrenti, C. Di Giacomo, M.L. Barcellona, M. Avitabile, A. Vanella. Published: Journal of Food Science Volume 67, Issue 1, pages 137–141, January 2002

This investigation into resveratrol as one of natures gout cures includes the following:

We have investigated the free radical scavenging capacity of resveratrol, its effects on xanthine oxidase (XO) activity, spontaneous membrane lipid oxidation, and DNA cleavage. Resveratrol showed a dose-dependent free radical scavenging activity, significant inhibition of XO activity…

Xanthine Oxidase (xanthine: oxygen oxidoreductase, E C 1.1.3.22) Activity Inhibition

Xanthine oxidase (XO) activity was evaluated spectrophotometrically by following the formation of uric acid … Different concentrations of resveratrol (50, 100, or 200 μM) were added to samples before enzyme addition and their effect on the generation of uric acid was used to calculate regression lines.

First 2 pages are shown as an image, full report is:

Resveratrol Xanthine Oxidase Inhibitor Investigation

Title:
Antioxidant Activity and Protective Effect on DNA Cleavage of Resveratrol.
Authors:
R. Acquaviva, A. Russo, A. Campisi, V. Sorrenti, C. Di Giacomo, M.L. Barcellona, M. Avitabile, A. Vanella.
Published:
Journal of Food Science Volume 67, Issue 1, pages 137–141, January 2002
Resveratrol Xanthine Oxidase Inhibitor Investigation

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GoutPal Sufferers Helped

GoutPal Sufferers Helped Chart

GoutPal consists of the main GoutPal.com reference website, and a support site, gout-pal.com, aka GoutPal Interactive.

GoutPal Interactive supports GoutPal.com by helping visitors use the website more effectively, but it has a wider role in helping gout sufferers. The selfless contributions of hundreds of fellow gout sufferers means millions of gout patients get help directly, or through better information on the main website.

At the moment, GoutPal.com is totally dependent on me, though I hope this will change in the near future. This chart clearly shows how important this is. Whilst the one-man GoutPal.com suffered the same fall as I did, the many-legged GoutPal Interactive showed continuous steady increases in the number of gout sufferers helped. A massive thank you to all GoutPal Interactive members.

GoutPal Sufferers Helped Chart

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Gout Sufferers Helped

Gout Sufferers Helped (click for chart details)

Visitors to my gout websites are a good indication of how many gout sufferers find help to overcome their pain. As the biggest gout support service, I was proud to be helping people on the road to Gout Freedom at the rate of one million per year, with plans to help many, many more.

Then came my fall from a balcony, and the resulting breaks meant a break from working on the website. My own fall mirrored by a fall in visitors.

But, let us look on the bright side. There is every indication that the decline is over. I still need a few months to complete physical recovery. My gout support websites need a few months to recover to one million per year and beyond. There is every indication of forthcoming success on both fronts

Gout Sufferers Helped Chart

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Gout Diet Question: What Are Purines?

Gout Diet Question Form Example: What Are Purines?

This completed gout diet question form is an example of how to ask a question related to food, drink, or lifestyle in the Gout Diet Forum.

Though the specific question is What Are Purines?, you should read the accompanying notes to learn how to ask much better gout questions.

Remember, the more interesting and unique your question is, the better answer you will get.

Also see the gout forum guidelines for more tips, and more ways to discuss gout.

Gout Diet Question Form Example: What Are Purines?

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Gout Laser Therapy

Laser Photograph

Low level laser is a good pain relief therapy for gout.

Laser Photograph Details

Thanks to Stéphane Magnenat at Flickr (flickr.com/photos/stephane_magnenat/2557475043/) for this cool laser light image. Although the image is not from actual kit used to treat pain, I like the combination of laser light and water – both having great benefits for gout sufferers.

Laser Photograph

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Urate Inflammation With Cartilage And Bone Destruction

Low-level in?ammation persists during the remissions of acute ?ares. Cytokines, chemokines, proteases, and oxidants involved in acute in?ammation contribute to chronic in?ammation leading to chronic synovitis, cartilage loss, and bone erosion. Monosodium urate (MSU) crystals are able to activate chondrocytes to release interleukin-1, inducible nitric oxide synthetase, and matrix metalloproteinases, leading to cartilage destruction. Similarly, MSU crystal activation of osteoblasts, release of cytokines by activated osteoblast, and decreased anabolic function contribute to the juxta-articular bone damage seen in chronic MSU in?ammation. IL = interleukin; iNOs = inducible nitrous oxide synthase; MMP-9 = matrix metalloproteinase-9; PGE2 = prostaglandin E2.

Diagram showing how, even though an acute flare might not be happening, uric acid crystals induce cellular changes that lead to permanent cartilage and bone damage.

Leave this page to browse the uric acid crystals in the Understanding Uric Acid Section

Urate Inflammation With Cartilage And Bone Destruction

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Progress Of Gout Flare

Recent advances in the understanding of acute gouty attack are illustrated (left). The attack is primarily neutrophil-dependent and initiated by the capacity of urate crystals to activate complements and to stimulate synovial lining cells and resident in?ammatory cells to induce a variety of in?ammatory mediators. As depicted (right), self-resolution of acute gout is mediated by several mechanisms, including coating of monosodium urate crystals with proteins and clearance by differentiated macrophages, neutrophil apoptosis, clearance of apoptotic cells, inactivation of in?ammatory mediators, and the release of anti-in?ammatory mediators. Dots represent humoral in?ammatory mediators, including cytokines and chemokines. Apo B = apolipoprotein B; Apo E = apolipoprotein E; C1q, C3a, C3b, C5a, C5b-9 complement membrane attack complex; IL = interleukin; LDL = low-density lipoprotein; LTB4 = leukotriene B4; MCP-1 = monocyte chemoattractant protein-1/CCL2; MIP-1 = macrophage in?ammatory protein-1/CCL3; MMP-3 = matrix metalloproteinase-3; NO = nitrous oxide; PAF = platelet-activating factor; PGE2 = prostaglandin E2; PLA2 = phospholipase A2; PPAR-? = peroxisome proliferator-activated receptor-? ligand; PPAR? = peroxisome proliferator-activated receptor-? ligand; TGF-? = transcription growth factor-? ; TNF-? =  tumor necrosis factor- ?; S100A8/A9 = myeloid-related protein; sTNFr = soluble tumor necrosis factor receptor.

Diagram showing the cellular effects of gout flares during and after an episode of acute gout.

Progress Of Gout Flare

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